T - Lymphocytic Leukemia Expresses Complex , Branched 0 - Linked Oligosaccharides on a Major Sialoglycoprotein , Leukosialin
نویسندگان
چکیده
Leukocytes express a major sialoglycoprotein, leukosialin, of which the apparent molecular weight (mol wt) can be variable according to the differences in 0-glycans attached to this molecule. In the present study, we analyzed the structures of 0-glycans attached to leukosialin present in various T-lymphocytic leukemia cells. T-lymphoid cells from patients with acute T-lymphocytic leukemia express a large amount of the branched hexasaccharides, NeuNAca2 -+ 3Galp1 -+ 3(NeuNAcaZ + 3Galpl -+ 4GlcNAcpl -+ 6)GalNAc. which are also expressed in activated normal T lymphocytes, but that are almost absent in resting normal T lymphocytes. T-lymphoid cells from patients with chronic T-lymphocytic leukemia, on the other hand. mainly express the tetrasaccharides
منابع مشابه
T - Lymphocytic Leukemia Expresses Complex , Branched 0 - Linked Oligosaccharides on a Major Sialoglycoprotein
Leukocytes express a major sialoglycoprotein, leukosialin, of which the apparent molecular weight (mol wt) can be variable according to the differences in 0-glycans attached to this molecule. In the present study, we analyzed the structures of 0-glycans attached to leukosialin present in various T-lymphocytic leukemia cells. T-lymphoid cells from patients with acute T-lymphocytic leukemia expre...
متن کاملT-lymphocytic leukemia expresses complex, branched O-linked oligosaccharides on a major sialoglycoprotein, leukosialin.
Leukocytes express a major sialoglycoprotein, leukosialin, of which the apparent molecular weight (mol wt) can be variable according to the differences in O-glycans attached to this molecule. In the present study, we analyzed the structures of O-glycans attached to leukosialin present in various T-lymphocytic leukemia cells. T-lymphoid cells from patients with acute T-lymphocytic leukemia expre...
متن کاملIsolation and characterization of leukosialin, a major sialoglycoprotein on human leukocytes.
A major sialoglycoprotein (previously called gp105) on the human erythroleukemic cell line K562 was purified, and specific antibodies were raised in a rabbit. A number of different hematopoietic cell lines belonging to erythroid, myeloid, T-lymphoid, and B-lymphoid cell lineages were found to possess glycoproteins that were immunoprecipitated by these antibodies. However, the apparent molecular...
متن کاملStructural variations of O-linked oligosaccharides present in leukosialin isolated from erythroid, myeloid, and T-lymphoid cell lines.
Structures of O-linked oligosaccharides of leukosialin isolated from K562 erythroid, HL-60 promyelocytic, and HSB-2 T-lymphoid cell lines were examined. Leukosialin was isolated by specific immunoprecipitation from cells which were metabolically labeled with [3H]glucosamine, and glycopeptides were isolated after Pronase digestion. O-Linked oligosaccharides were released by alkaline borohydride ...
متن کاملAsn-linked oligosaccharides from metastatic tumour cells
Increased branching at the trimannosyl core of 'complex-type' Asn-linked oligosaccharides has been observed in both human and murine tumour cells, and appears to be associated with enhanced metastatic potential in several murine tumour models [Dennis, Laferte, Waghorne, Breitman & Kerbel (1987), Science 236,582-585]. The lectin leucoagglutinin (L-PHA) requires the -GlcNAc/31 -6Mana 1 -6Man-link...
متن کامل